16. Polyadenylation (polyA tail) is usually found in which type of RNA molecules?
(A) rRNA
(B) tRNA
(C) siRNA
(D) mRNA

17. Sir Frederick Sanger is a British scientist who was awarded Nobel Prize twice. Which of the following statements of him is   NOT   true? (A)He determined the structure of Insulin. (B)He developed a DNA-sequencing method. (C)He established the hybridoma technology to produce monoclonal antibodies. (D)Some of his graduate students also received the Nobel Prize

18. Please select the appropriate primers to amplify only the coding region of the following gene   EXCLUDING   the stop codon.5' GCTCGATGGATCCAAACACTGTGTCAAGCTTTCAGGACATACTGCTGAGGATGTCAAAAATGC AGTTGGAGTCCTCATCGG AGGACTTGAATGGAATGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAG AAGCAGTAATGAGAATGGGAG ACCTCCACTCACTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTCAGAAGTTTGA AGAAATAAGATGGTTGATTGAAG AAGTGAGACACAAACTGAAGATAACAGAGAATAGTTTTGAGCAAATAACATTTATGCAAGCC TTACATCTATTGCTTGAAGTGG AGCAAGAGATAAGAACTTTCTCGTTTCAGCTTATTTAG 3' (A)Primer 1: 5' GCTCGATGGATCCAAACACTGTGTC 3'; Primer 2: 5' CTTTCTCGTTTCAGCTTATTTAG 3' (B) Primer 1: 5' ATGGATCCAAACACTGTGTCAAGC 3'; Primer 2: 5' CTTTCTCGTTTCAGCTTATTTAG 3' (C) Primer 1: 5' GCTCGATGGATCCAAACACTGTGTC 3'; Primer 2: 5' CTAAATAAGCTGAAACGAGAAAGTTC 3' (D)Primer 1: 5' ATGGATCCAAACACTGTGTCAAGC 3'; Primer 2: 5' AATAAGCTGAAACGAGAAAGTTC 3'

19. The consensus sequence for poly（Ａ） addition is (A) The site of poly（Ａ） tail addition (B) AAUAAA (C) Downstream of the cleacage site (D) None of the above

20. Alternative splicing can result in which of the following gene modifications,   EXCEPT   (A) Production of different mRNA molecules from the same gene (B) Production of different proteins from the same gene (C) Production of same peptide domain (D) Production of two or more proteins with different functional domains

21. Which of the following enzymes is   NOT   required during mammalian DNA replication? (A) Primase (B) DNA ligase (C) DNA helicase (D) T4 DNA polymerase

22. All of the followings are involved in translation of protein in a cell   EXCEPT   (A) rRNA (B) siRNA (C) mRNA (D) tRNA

23. Which of the followings is a DNA molecule? (A)(B)(C)(D)

24. Which of the following descriptions of "virus" is   INCORRECT  ? (A) A virus is a small infectious agent that can replicate only inside the living cells of an organism. (B) A virus is composed of protein coat and nucleic acid. (C) The genetic material of a virus is either a double strand DNA or a single strand RNA. (D) During virus replication, some virus particles may not contain viral genome.

複選題25. Which of the following methods is used to detect the DNA-protein interaction? (A) DNA cloning (B) Electrophoretic Mobility Shift Assay (C) MALDI-TOF analysis (D) Yeast-two-hybrid system

26. In which phase of the cell cycle does the DNA synthesis occur? (A) G1 (B) S (C) G2 (D) M

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