19. Which is not the loss-of-function method to study a gene function?
(A) RNA interference
(B) Clustered regularly interspaced short palindromic repeats
(C) Zinc finger nuclease
(D) Gene targeting
(E) Finger printing

20. What is incorrect about reverse transcriptase (RT)? (A) Telomerase is one kind of RT. (B) It is found in retrovirus. (C) It synthesizes DNA from 3' to 5' (D) It needs a primer and template. (E) It is essential for cDNA synthesis.

21. Which of the following statements regarding Vesicle transport is incorrect? (1) Vesicle budding recruits proteins that are needed for subsequent selective vesicle targeting and fusion. (2) Clathrin coated vesicles are pinched off in a dynamin-mediated process.  (3) Vesicle transport along actin filaments is driven by myosin II. (A) (1) (B) (2) (C) (3) (D) (1) and (2) (E) (2) and (3)

22. Which of the following statements regarding Golgi apparatus is correct? (1) Each cisterna or region of the Golgi contains different protein modification enzymes.  (2) The Golgi enzymes catalyze the addition of sugars sulfate groups and the addition of phosphate groups onto cargo proteins. (3) Some Golgi-mediated modifications act as signals to direct the proteins to their final destinations within cells, including the lysosome and the plasma membrane. (A) (1) (B) (2) (C) (3) (D) (1) and (2) (E) All are correct.

23. Which of the following statements regarding extracellular vesicles (EVs) is incorrect? (A) Exosomes and microvesicles both are extracellular vesicles (EVs) but differ in their cellular origin. The exosomes bud off from the cell membrane but MVs have endocytic origin. (B) EVs contain mRNAs and miRNAs and those EV-associated mRNAs and miRNAs can be functionally transferred to recipient cells. (C) Tumor cells have been shown to exploit EVs to contribute to their progression by inactivating T lymphocytes or natural killer cells as well as promoting differentiation of regulatory T lymphocytes to suppress immune reactions. (D)Several pathogenic proteins such as prions and β-amyloid peptides have also been reported to exploit exosomes in order to propagate to other cells. (E) None is incorrect

24. Which of the following statements regarding stem cells is correct? (1) Pluripotent stem cells have the potential to become an entire organism. (2) Totipotent stem cells can form any of the three embryonic germ layers, but cannot give rise to an entire organism. (3) Multipotent cells can develop into more than one cell type, but are more limited than pluripotent cells. (4) Totipotent, pluripotent or multipotent stem cells are cells that have the capacity to self-renew by dividing asymmetrically. (A) (1) and (2) (B) (2) and (3) (C) (3) and (4) (D) (1) and (4) (E) (2) and (4)

25. Activation of the notch receptor by its ligand, delta, in neighboring cell populations defines patterns of distinct differentiated cell types among cells that have the potential to adopt the same fate. What is this process called? (A) Lateral inhibition (B) Neurulation (C) Invagination (D) Gastrulation (E) None of above

26. Which of the following statements regarding heterotrimeric G protein and G-protein-mediated signaling is correct? (1) The alpha subunit of heterotrimeric G protein (G-alpha), belong to the larger group of enzymes called GTPase. (2) There are several different classes of heterotrimeric G-proteins that are defined by their different G-alpha, G-beta or G-gamma subunits. (3) G-protein coupled receptors (GPCRs) associate with heterotrimeric G-proteins. Upon ligand binding to GPCRs, G-alpha hydrolyze bounded GTP to GDP and dissociate from the other subunits of heterotrimeric G-proteins. (A) (1) and (2) (B) (1) and (3) (C) (2) and (3) (D) All are correct (E) None is correct

27. Which of the following statements regarding immune response is correct? (1) In cellular immunity, T lymphocytes, including cytotoxic T lymphocytes and killer T cells are responsible for the recognition and killing of foreign invaders.  (2) Killer T lymphocytes have an especially low count in a patient with advanced AIDS. (3) Histamine is produced by host cells infected by viruses.  (4) Immunological memory is due to long lived B cells that secrete a specific antibody. (A) (1) and (2) are correct (B) (1) and (3) are correct (C) (1) and (4) are correct (D) (1), (2) and (4) are correct (E) (1), (3) and (4) are correct

28. The order for the construction of a cDNA fragment from mRNA is to: (A) treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT. (B) digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase. (C) bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase. (D) bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase. (E) None of above is correct

29. Which one is NOT involved in COPII vesicle formation? (A) GTPase Sar1 (GTP-bound state) (B) guanine nucleotide exchange factor (GEF) Sec12 (C) Coat Sec23/Sec24 heterodimer. (D) Outer coat Sec13/Sec31 heterotetramer (E) From Golgi to lysosome

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