20.各機關新僱用之駕駛，應該具備之要件，以下那一項不正確？
(A)須品德端正
(B)須具有合格之普通駕駛執照→職業
(C)無不良嗜好
(D)須有公立醫院之體格檢查證明

21.公務載客用小型車輛至少屆滿幾年時，按規定應辦理財產報廢? (A)10年 (B)12年 (C)15年 (D)18年

22.依照車輛管理手冊規定，在肇事處放置顯明標誌的原則為何？ (A)肇事處前方100公尺 (B)肇事處後方100公尺 (C)肇事處前後100公尺 (D)肇事地點兩端適當距離處

23.依據車輛管理手冊，機關雇用駕駛人力所應符合的規定，下列何者錯誤？ (A)須品德端正，無不良嗜好 (B)須有公立醫院之體格檢查證明 (C)須1年內無交通違規紀錄 (D)須查明其過去工作情形及離職原因

複選題24.下列有關油料管理之敘述，何者正確？ (A)車輛用油範圍只包含汽油、柴油等各種燃料用油 (B)車輛用油一律依政府採購法規定購買 (C)車輛之耗油標準，應由各機關會同出納、會計人員依車輛一般政常狀況實地勘測訂定 (D)燃料用油除自設加油設施者外，以不備存為原則

複選題25.有關各機關車輛之調派規定，以下那一項敘述比較不正確？ (A)上班交通車若遇特殊事故無法準時抵達各站，候車人當天可以請公假 (B)交通車行駛路線及停車站，由事務單位規定 (C)車輛管理人員應該按照各單位送到派車單先後緩急依次調派 (D)下班後，如遇緊急事件，需用車輛單位得逕行與車輛管理人員聯絡後派遣之

1.Enhancer function underlies regulatory processes by which cells establish patterns of gene expression. Which of the following descriptions about enhancer is not correct? (A) Enhancer works as cis-regulatory elements to mediate spatiotemporal control of gene expression. (B) Enhancers can be identified using a reporter gene. Once a reporter gene is integrated near an enhancer, its expression reflects the expression pattern. (C) Next-generation sequencing (NGS)-based approach called RNase-seq enables identification of enhancers. (D) Enhancer help recruit RNA polymerase II to promoters and can attract various chromatin-modifying enzymes to DNA. (E) Enhancer is distal sequences that lie upstream or downstream of the core promoter and recruit transcription factors

2.The DNA polymerases are the workhorses for replication of genomic information in living cells. Which of the following descriptions about their biotechnological applications is not correct? (A) During polymerase chain reaction (PCR), DNA polymerases often extend misprimed targets and primer-dimers, which are common sources of nonspecific amplification. (B) During PCR, hot-start DNA polymerases reduce nonspecific amplification, increase yields, and allow convenient room temperature setup for high-throughput applications. (C) Although archaeal DNA polymerases are extremely heat-stable, they may bc slow in synthesizing DNA due to lower processivity. (D) High-fidelity DNA polymerases are enzymes with strong proofreading activity based on their 3'→5' exonuclease activity. (E) DNA polymerases can be engineered with high specificity by introducing a strong DNA-binding domain of another protein without compromising polymerase activity.

3. Which of the following components or events is not involved in epigenetic gene regulation? (A) Histone acetylation (B) Heavy methylation of promoter regions (C) Ubiquitin-mediated degradation of transcription factors (D) Nucleosome sliding (E) Chromatin opening and closing

4. Agrobacterium-mediated T-DNA delivery of CRISPR/Cas9 expression cassettes is an advanced tool for genome editing in plants. Which of the following descriptions about this technique is not correct? (A) Only the T-DNA region is transferred from agrobacterium into the host plant's nuclear DNA genome. (B) The virulence region of the Ti plasmid encodes proteins to form a membrane- spanning DNA transporter called the type IV secretion system (T4SS). (C) CRISPR/Cas9 cleaves foreign DNA via two components, Cas9 and sgRNA. (D) The sgRNA is a synthetic RNA containing a 5'-end with a 20-nt sequence that acts as a guide sequence to identify the target sequence accompanied by a protospacer adjacent motif (PAM) sequence. (E) When a template is present, nonhomologous end-joining (NHEJ) can be activated and results in gene replacement or knock-in.

5. Which of the following statements about small RNA is not correct? (A) Small RNAs are short, non-coding RNA molecules that can regulate gene expression. (B) Small RNAs can regulate gene expression in the nucleus via chromatin- dependent gene silencing. (C) Most small interfering RNAs (siRNAs) are generated through multiple processing steps from longer primary transcripts produced by RNA polymerase II. (D) Most microRNAs (miRNAs) form imperfectly complementary stem-loop structures, pair imperfectly with sites in the 3' untranslated region of their target mRNAs. (E) Both miRNAs and siRNAs are produced from longer RNA precursors through the activity of ribonuclease IlI-like nucleases called Dicer in animals and DICER- LIKE (DCL) in plants.

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