26. All repressors and activator proteins share which of the following structural features:
(A) A repression domain
(B) An activation domain
(C) A ligand-binding domain
(D) A DNA-binding domain

27. What determines the time and place that a certain gene is transcribed in the cell? (A) The type of cis-regulatory sequences associated with it (B) The relative position of cis-regulatory sequences associated with it (C) The arrangement of various cis-regulatory sequences associated with it (D) All of the above

28. Which of the following DNA-binding motifs uses ẞ sheets to recognize DNA bases? (A) The helix-turn-helix motif (B) The leucine zipper (C) The zinc finger motif (D) None of the above

29. Under which of the following conditions is the Lac operon in Escherichia coli fully turned on? (A) Low glucose and lactose levels (B) Low glucose but high lactose levels (C) High glucose but low lactose levels (D) High glucose and lactose levels

30. The Trp operon in Escherichia coli encodes the components necessary for tryptophan biosynthesis. In the presence of the amino acid in a bacterium, (A) the tryptophan operator is bound to the tryptophan repressor. (B) the tryptophan repressor is bound to bacterial RNA polymerase. (C) the expression of the tryptophan repressor is shut off. (D) the operon genes are expressed. 

1. If we have one DNA sequence with a 5-minutes PCR cycle time. After 1 hour, how many DNA sequence can we get? (A) 1024 (B) 2048 (C) 3072 (D) 4096

2. Which statements of SDS PAGE is correct? (A) SDS acts as a detergent and attaches to the protein, so the protein has positive charge. (B) Direction of protein movement is from positive charge (+) to negative charge (-). (C) APS (ammonium persulfate) as a buffer of SDS PAGE.(D) High molecular weight protein would stay in the upper section of the SDS PAGE.

3.   A   is a common experimental method to detect protein in a biological sample.   B   can investigate DNA in the biological sample. Dr. Kary Banks Mullis builts a method   C   which can ampilify DNA in a short period of time. Based on these description, what are methods   A  ,   B   and   C  ? (A) A: Southern blot, B: Eastern blot, C: CPR. (B) A: Southern blot, B: Western blot, C: CPR. (C) A: Western blot, B: Northern blot, C: PCR. (D) A: Western blot, B: Southern blot, C: PCR.

4. Which statement is correct? (A) Nucleoside contains nitrogen base, pentose sugar and phosphate group (B) Watson and Crick discovered RNA is a double helix structure (C) Gout patients should decrease ingestion of high purine food to release symptom (D) DNA is composed of A, T, G, C. A is a kind of pyrimidine.

5. 2D-PAGE is a method to investigate protein. Differential migration of analytes in 2D-PAGE let us measure protein expression difference between two samples. The rate of migration does not depend on? (A) Isoelectric point (B) Fluorescence (C) Protein size (D) Molecular weight

6. Which two amino acids can bind together and form disulfide bond? (A) Methionine and cysteine. (B) Serine and threonine. (C) Lysine and tyrosine. (D) Cysteine and cysteine.

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