27. Which of following methods could be used for studying protein-protein interactions?
(A) microarray
(B) ChIP-Seq
(C) co-immunoprecipitation
(D) all of them

28. A researcher identified a sequence of a novo gene from an unknown animal. What is the easiest way to guess the function of this novo gene? (A) put this gene into bacteria (B) BLAST this sequence (C) delete this gene in this animal (D) isolate the gene product

29. Which of the following factors is involved in proofreading during transcription in prokaryotes? (A) GreB (B) NusA (C) sigma-factor (D) rho-factor

30. A prokaryote experiment was conducted to determine the hairpin loop region in termination. Two DNA templates were prepared and used in an in vitro transcription assay. The samples were analyzed for release of the RNA polymerase. Construct 1 - hairpin sequence removed; Construct 2 - two copies of hairpin sequence inserted; Construct  3 - normal DNA with only one hairpin sequence The data from this experiment suggest that (A) hairpin loops are not required for termination. polymerase. (B) two hairpin loops rapidly destabilize the (C) the data are inconclusive. (D) additional hairpin loops increase the stability of the RNA:DNA hybrid.

31. Which of the following techniques is most useful in determining if RNA polymerase has initiated transcription from the lac DNA template in prokaryotes? (A) Southern analysis (B) RACE (C) run-off transcription assay (D) DNA fingerprinting

32. In the process of creating a trp operon mutant, the region for the riboswitch was accidentally deleted. Which of the following would be a likely observation in the transcription assays? (A) RNA polymerase cannot bind to the promoter. (C) Attenuation would be impaired. (B) The operon is permanently inhibited. (D) Termination would be triggered.

33. Which of the following sigma specificity factors is involved in middle gene transcription during SPO1 phage infection of bacteria? (A) gp28 (B) gp33 (C) gp43 (D) host sigma

34. Which of the following proteins is not involved the lytic phase of 2-infection of bacteria? (A) CIII (B) S10 (C) NusA (D) NusG

35. All of the following contain helix-turn-helix motifs except (A) repressor (B) tryptophan (C) Cro (D) CAP

36. Which of the following is NOT a part of the core class II promoter in eukaryotes? (A) TATA box (B) upstream element (C) BRE (D) DPE

37. The CCAAT boxes are bound by which of the following? (A) Sp1 (B) DPE (C) TBP (D) CTF

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