29. The usage of monoclonal antibodies is   NOT   required for
(A) DNA agarose gel analysis
(B) Enzyme-linked immuno-sorbent assay
(C) Immunohistochemistry
(D) Western blotting

30. In order to examine the size of a protein, the most proper gel material is (A) agarose (B) nitrocellulose (C) polyacrylamide (D) starch

31. Which of the following descriptions is correct for DNA polymerase? (A) binds to promoter regions (B) binds to enhancer regions (C) usually functions well at 4°C (D) uses RNA as template 

32. Which of the following statements is correct regarding splicing? (A) smURFs are used to recognize the poly A tail and initiate slicing (B) snRNPs are the protein product of U rich RNAs that partially complement the 5' splice junction. (C) CPSFs are products of RNA sequences designed to recognize the 3'OH at the splice site. (D) PABIII recognizes the A rich sequences at the 5' end of the splice site.

33. The function of the TATA box is (A) to form a hairpin for RNA transcription termination. (B) to terminate active RNA transcription. (C) to recognize the subunit and correctly line up the RNA polymerase. (D) to provide a binding location for the Rho protein.

34. Class I and Class II aminoacyl-tRNA synthetases differ in their I. structural motifs.  II. anticodon recognition. III. site of aminoacylation.  IV. amino acid specificity. (A) I only (B) II only (C) II,III, IV (D) I, II, III, IV

35. Which of the following is   NOT   associated with eukaryotic translation initiation? (A) Shine-Dalgarno sequence (B) a formylated Met--tRNA met (C) base pairing between the mRNA and 16S rRNA (D) all of the above

36. During translation, translocation of the ribosome is driven by which of the following? (A) GTP-GDP + P (B) ATP ADP + P＜sub＞i＜/sub＞ (C) ATP AMP + PP＜sub＞i＜/sub＞ (D) Release of the tRNA in the P site

37. Which of the following is correct? (A) Transfer of genetic information via direct cell-cell contact is called transfection. (B) Specialized transduction can be carried out by any bacteriophage. (C) Transformation has been observed in only a limited number of bacteria in nature, but we have found ways to force the process upon other species in the laboratory. (D) All of above

38. Which set of forward and reverse primers will be appropriate to amplify the following gene? ATGGATCCAAACACTGTGTCAAGCTTTCAGGACATACTGCTGAGGATGTCAAAAATGCAGTT GGAGTCCTCATCGGGGGACTTGAATGGAATGATAACACAGTTCGAGTCTCTGAAACTCTACA GAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACTCACTCCAAAACAGAAA CGAGAAATGG GGAACAATTAGGTCAGAAGTTTGAAGAAATAAGATGGTTGATTGAAGAA GTGAGACACAAACTGAAGATAACAGAGAATAGTTTTGAGCAAATAACATTTATGCAAGCCTT ACATCTATTGCTTGAAGTGGAGCAAGAGATAAGAACTTTCTCGTTTCAGCTTATTTAG (A) ATGGATCCAAACACTGTGTCAAGC; CTTTCTCGTTTCAGCTTATTTAG (B) ATGGATCCAAACACTGTGTCAAGC; CTAAATAAGCTGAAACGAGAAAG (C) GCTTGACACAGTGTTTGGATCCATT; CTAAATAAGCTGAAACGAGAAAG (D) GCTTGACACAGTGTTTGGATCCATT; CTTTCTCGTTTCAGCTTATTTAG 

39. Which of the following techniques is used to identify protein-protein interactions? (A) Northern blot analysis (B) Southern blot analysis (C) Western blot analysis (D) Yeast-two-hybrid system

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