29. Which one of the following methods can be used to locate genes in chromosomes?
(A) FISH
(B) Southern blot
(C) Northern blot
(D) EMSA

30. Nine promoters (promoter 1-7) are individually cloned into the CAT (chloramphenicol acetyl transferase) reporter plasmid, and each plasmid is transfected into cells, followed by assay of the CAT activity using thin-layer chromatography. The result is shown below. According to this result, which promoter has the strongest activity? (A) Promoter 1 (B) Promoter 2 (C) Promoter 4 (D) Promoter 6

31. What is the major determinant in the measurement of the size of a DNA fragment during agarose gel electrophoresis? (A) charge of a DNA fragment (B) molecular weight of a DNA fragment (C) charge to molecular weight ratio of a DNA fragment (D) friction force between DNA fragment and agarose gel

32. To ascertain which genes are actively transcribed in a given cell under a specific condition (such as stimulation by a growth factor), the method that can be used to address this question is: (A) Run-off transcription (B) Nuclear run-on transcription (C) 5'-RACE (D) S1 mapping

33. DNase footprinting is usually used to: (A) determine the sequence of DNA fragment (B) locate a protein binding site in a DNA fragment (C) locate the 5'-end of a DNA fragment (D) locate the 3'-end of a DNA fragment

34. Which of the following can not help you discover a tumor suppressor gene? (A) Cell transformation assay using NIH-3T3 cells transfect with random DNA fragments from human cancer cells. (B) Positional cloning of the genes causing rare familial cancers (C) Defining chromosomal locations commonly deleted in tumor cells (D) Testing tumors for mutations in genes known to be involved in cell cycle regulation.

35. The primary RNA transcript of the chicken ovalbumin gene is 7700 nucleotides long, but the mature mRNA that is translated on the ribosome is 1872 nucleotides long. This size difference occurs primarily as a result of: (A) capping (B) cleavage of polycistronic mRNA (C) removal of poly A tails (D) splicing

36. RNAs that catalyze biological reactions, such as self-splicing introns, are known as: (A) enzymes (B) spliceosomes (C) ribozymes (D) lariats

37. Which statement is not true about nucleic acid hybridization? (A) it depends on complementary base pairing (B) a polysaccharide can hybridize with a DNA strand (C) a DNA strand can hybridize with another DNA strand (D) an RNA strand can hybridize with a DNA strand

38. Promoters for eukaryotic mRNA synthesis: (A) are more complex than prokaryotic promoters (B) can require binding of multiple transcription factors to form a transcription complex (C) have specific DNA sequences such as the "TATA" box that are recognized by proteins (D) all of above

39. mRNA will form hybrids only with the coding strand of DNA because: (A) DNA will not reanneal at high temperatures (B) the salt concentration will affect DNA reannealing (C) DNA will not reanneal at low temperatures (D) RNA:DNA hybridization follows the base-pairing rules

115年 - [無官方正解]115 國立清華大學_碩士班入學考試試題_生命科學暨醫學院甲組(生物與醫學科學組)：分子生物學#142697

114年 - [無官方正解]114 國立中正大學_碩士班招生考試試題_生物醫學科學系：分子生物學#138414

114年 - [非官方正解]114 國立清華大學_碩士班入學考試試題_生命科學暨醫學院甲組(生物與醫學科學組)：分子生物學#132699

113年 - [無官方正解]113 國立中正大學_碩士班招生考試試題_生物醫學科學系：分子生物學#136961

113年 - [非官方正解]113 國立成功大學_碩士班招生考試_藥理學研究所 :分子生物學#126004

113年 - [非官方正解]112 國立臺灣大學_碩士班招生考試：分子生物學(B)#118906

112年 - [非官方正解]112 國立臺灣大學_碩士班招生考試：分子生物學(C)#120020

112年 - [非官方正解]112 國立臺灣大學_碩士班招生考試：分子生物學(B)#120007

112年 - [非官方正解]112 國立臺灣大學_碩士班招生考試：分子生物學(D)#118608

112年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(A)#118594