7. When expressing a eukaryotic protein in a bacterial host, why is it critical to use cDNA rather than genomic DNA? (4 pts)

8. In quantitative PCR (qPCR), why do we focus on the Ct value (Threshold Cycle) rather than the final amount of product at the end of the reaction? (3 pts) Briefly define what the Ct value represents. (3 pts)

9. You observe that adding DNase to a bacterial culture prevents the bacteria from acquiring a new hereditary trait. Based on this observation, which gene transfer mechanism is occurring (Transformation, Transduction, or Conjugation)? Explain your reasoning. (4 pts)

10. Why is a frameshift mutation located at the beginning of a coding region generally more detrimental to protein function than a missense mutation? Briefly explain the reason. (4 pts)

(1) The number of moles of aspirin in one tablet.

(2) The number of aspirin molecules in one tablet.Report both answers to 3 significant figures using scientific notation.

35. The Ksp values of three sulfide salts are given below:(A) CuS: 8.5 × 10⁻⁴⁵; (B) Ag₂S: 1.6 × 10⁻⁴⁹; (C) Bi₂S₃: 1.1 × 10⁻⁷³Arrange the salts in order of decreasing molar solubility:_____> _______>_______.

36. Silver crystallizes in a cubic closest packing (ccp) structure, which corresponds to a face-centered cubic (fcc) unit cell, as shown. The atomic radius of silver is r = 1.44 Å. Calculate the density of silver by completing the following steps. Report all calculated values to 3 significant figures.(1) There are effectively _ silver atoms in one unit cell.(2) The edge length of the unit cell, d, is _ Å.(3) The volume of the unit cell is _ cm³.(4) The total mass of silver in one unit cell is _ g.(5) The density of solid silver is _ g/cm³.

37. Pure water freezes at 0.00°C under 1 atm, and its molal freezing-point depression constant is Kƒ = -1.86°C kg mol⁻¹. A solution is prepared by dissolving 14.84 g of a non-electrolyte in 291.7 g of water. The freezing point of the solution is -0.276°C. Calculate the molar mass of the solute. Report your answer to 3 significant figures.

38. The complex ion [CoBr₄]²⁻ has a tetrahedral geometry. Answer the following questions.(1) How many 3d electrons are present in the cobalt center?(2) Draw the crystal-field splitting diagram for the 3d orbitals in a tetrahedral ligand field and indicate the electron configuration by placing the electrons in the appropriate orbitals.

二、簡答題 1. The primary challenge in oncological pharmacology is achieving site-specific delivery while minimizing systemic toxicity. You are tasked with designing a drug delivery vehicle for a novel lung cancer therapeutic that must bypass the systemic circulation and selectively enter the cytoplasm of malignant lung epithelial cells. Discuss the molecular mechanisms you would exploit to ensure this specificity. In your response, compare the efficacy of targeting the extracellular matrix (ECM) with that of receptor-mediated endocytosis. Why is the mere chemical property of the drug (such as hydrophobicity) insufficient for achieving high tissue specificity in a complex multicellular organism?

115年 - [無官方正解]115 國立清華大學_碩士班入學考試試題_生命科學暨醫學院甲組(生物與醫學科學組)：分子生物學#142697

114年 - [無官方正解]114 國立中正大學_碩士班招生考試試題_生物醫學科學系：分子生物學#138414

114年 - [無官方正解]114 國立清華大學_碩士班入學考試試題_生命科學暨醫學院甲組(生物與醫學科學組)：分子生物學#132699

113年 - [無官方正解]113 國立中正大學_碩士班招生考試試題_生物醫學科學系：分子生物學#136961

113年 - 113 國立成功大學_碩士班招生考試_藥理學研究所 :分子生物學#126004

113年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(B)#118906

112年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(C)#120020

112年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(B)#120007

112年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(D)#118608

112年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(A)#118594