105年 - [無官方正解]105 國立嘉義大學_碩士班(乙組)招生考試試題_應用化學系：生物化學#147050

1. Which of the following can be used as is a reduced form coenzyme? (A) FAD (B) FMN (C) NADP⁺ (D) NADH (E) CoQ

2. Watson and Crick proposed the B-form double helix model for DNA structure. What is the best nucleotide to be complementary to cytosine, C? (A) G (B) T (C) U (D) C (E) A

3. What function do kinase enzymes catalyze? (A) phosphorylation (B) methylation (C) acetylation (D) ubiquitination (E) reduction

4. In general, a newly synthesized messenger RNA, called primary RNA or pre-mRNA, contains introns and exons. What is the process in which the introns are removed and the exons are joined to form a matured mRNA? (A) signal transduction (B) translocation (C) transcription (D) translation (E) splicing

5. Glucose is ultimately oxidized to CO₂ and H₂O via TCA cycle (Krebs cycle) in cells. What intermediate will glucose be degraded into before entering the TCA cycle? (A) malate (B) isocitrate (C) succinate (D) acetyl-CoA (E) citrate

6. In study of enzyme kinetics, turnover number is defined as the number of substrate molecules converted to product in a given time on a single enzyme molecule when enzyme is saturated with substrate. Which of the following terms is equivalent to turnover number? (A) Km (B) Kd (C) Ka (D) Vmax (E) Kcat

7. When one says a protein contain -helix structure, the -helix structure is one feature of (A) primary structure (B) secondary structure (C) tertiary structure (D) quaternary structure (E) Z-form structure.

8. Where do the light reactions take place (A) mitochondrion (B) cytosol (C) nucleus (D) lysosome (E) chloroplast

二、 問答題 60 分(每題15 分)
1. Please give the English names of 20 amino acids.

2. Clearly describe the processes how ATP is produced from NADH or FADH2 in mitochondria.

3. How proteins are translocated from cytosols to the endoplasmic reticulum?

4. The process of initiation step for protein translation in prokaryotic and eukaryotic cells is similar but somewhat different. Explain how ribosomes bind to mRNA and reach to the start codon of an mRNA in prokaryotic and eukaryotic cells, respectively.