所屬科目:研究所、轉學考(插大)◆生物化學
1. Watson and Crick proposed the B-form double helix model for DNAstructure. What is the best nucleotide to be complementary tothymine, C?(A) guanine (B) cytosine (C) adenine (D) Uracil (E)pyrimidine
2. In general, a newly synthesized messenger RNA, called primaryRNA or pre-mRNA, contains introns and exons. What is the process inwhich the introns are removed and the exons are joined to form amatured mRNA?(A) signal transduction (B) translocation (C) transcription(D) translation (E) splicing
3. Where are most of proteins translated?(A) trans-Golgi (B) cytosols (C) plasma membrane(D) cis-Golgi (E) peroxisome
4. In aerobic organism, glucose are ultimately oxidized to CO2 andH2O via TCA cycle (Krebs cycle). What compound will glucose bedegraded and further catalyzed to before entering the TCA cycle?(A) acetyl-CoA (B) isocitrate (C) succinate (D) malate (E)citrate
5. In study of enzyme kinetics, turnover number is defined as thenumber of substrate molecules converted to product in a given timeon a single enzyme molecule when enzyme is saturated with substrate.Which of the following terms is equivalent to turnover number?(A) Km (B) kd (C) Ka (D) Vmax (E) kcat
6. When one says a protein contain -helix structure, the -helixstructure is one feature of(A) primary structure (B) secondary structure (C)tertiary structure(D) quaternary structure (E) super-molecular structure.
7. Which of the following coenzymes is a reduced form?(A) NAD+ (B) FMN (C) NADP+ (D) FAD (E) NADH.
8. Which of the following compounds is not a carbohydrate?(A) glucose (B) fructose (C) ribose (D) sucrose (E)adipose
9. Which of the following complexes is not involved in the electrontransport and ATP synthesis in the thylakoid during the photosynthesis(A) PSII complex (B) cytochrome b6f complex (C) PSIcomplex(D) PSIII complex (E) proton translocating ATP synthase
10. There are approximately 57,000 genes in rice. How many genesare in human beings?(A) about 10,000 (B) about 20,000 (C) about 50,000(D) about 60,000 (E) about 100,000
11. Please write the names of 20 amino acids in mammalian proteins
12. What is the DNA melting point?
13. What is SDS-PAGE?
14. Please explain allosteric regulation and feedback inhibition,respectively.
15. Draw a plausible hydrogen-bonding structure between the sidechain of threonine andlysine. (more than one answer is possible)