11. The main blood buffer system is
(A)
(B)
(C)
(D) none of the above

12. The Bohr effect for oxygen binding states that (A) Mb binds oxygen more tightly than Hb. (B) Hb will bind oxygen very tightly when the CO2 concentration is high. (C) as the pH goes down, Hb binds oxygen less tightly. (D) Hb's ability to bind oxygen increases with higher oxygen concentration.

13. Which would be best to separate positively charged proteins? (A) Gel filtration (B) Affinity chromatography (C) Cation exchange (D) Anion exchange (E) Cation or anion exchange

14. Determination of the sequence of amino acids in a peptide is done by (A) x-ray crystallography (B) Edman degradation (C) treatment with cyanogen bromide (D) trypsin hydrolysis

15. If a protein with the sequence PQRKYPIG is treated with trypsin, what will the products be? (A) PQR         KYPIG (B) PQRK     YPIG (C) PQR     K     YPIG (D) PQ       R     KPIG0

16. If a protein with the sequence FEWPRQVDMARINE is treated with chymotrypsin, what will the products be? (A) F EW PRQVMARINE (B) FE WPRQVD MARINE (C) FEWPR QVDMAR INE (D) FEWPRQVDM ARINE

17. If the y-intercept of a Lineweaver-Burk plot = 1.91 (sec/millimole) and the slope = 75.3 L/sec, KM equals: (A) 0.0254 millimolar (mM). (B) 0.523 millimolar (mM). (C) 5.23 millimolar (mM). (D) 39.4 millimolar (mM). (E) 75.3 millimolar (mM).

18. Which of the following is more likely to inhibit regulatory subunits of an allosteric enzyme? (A) A competitive inhibitor (B) A non-competitive inhibitor (C) An irreversible inhibitor (D) All of these are equally likely to inhibit a regulatory subunit

19. If an inhibitor changes the slope of the Lineweaver-Burk graph, but not the y-intercept, it is this type of inhibition: (A) Competitive. (B) Non-competitive. (C) Mixed Inhibition (uncompetitive inhibition). (D) You cannot tell from the data given. (E) More than one answer is correct.

20. Many metabolic pathways involve multistep reactions. Consider the following pathway.Which of the following would be an example of feedback inhibition? (A)the product of the final reaction, F, interacting with E1. (B) F interacting with an allosteric site in E4. (C) B interacting with an allosteric site in E1. (D) all of the intermediates or products in the reaction interacting with the active site in E1

21. The oligonucleotide AGGUCCAUUGAAp, is best described as (A) an RNA oligonucleotide with a 3'phosphate (B) an RNA oligonucleotide with a 5'phosphate (C) a DNA oligonucleotide with a 3'phosphate (D) a DNA oligonucleotide with a 5'phosphate

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