15. A close circular plasmid DNA has a length of 3000 base pairs. What is its linking number in relaxed form?
(A) Around 100;
(B) Around 300;
(C) Around 30;
(D) 0

16. Which of the following description about enzymes is   incorrect  ? (A) According to the nomenclature system of the systemic name, kinases are belonged to the class of transferases. (B) Coenzymes are also substrates of enzymes. (C) Acylation is one mechanism leading to the formation of a different isoforms of an enzyme. (D) Different isoenzymes must have the same catalytic properties for the same reaction.

17. Which of the following statement is correct about reversible inhibition of enzymatic reactions? (A) Both Vmax and Km are reduced in uncompetitive inhibition. (B) A competitive inhibitor binds at a site on the enzyme molecule other than the substrate-binding site. (C) Km in competitive inhibition is unaffected. (D) At a high concentration of the substrate, the inhibitory effect of the noncompetitive inhibitor can be overcome.

18. Which of the following vitamins are cofactors required for the enzymatic reactions involved in the conversion of homocysteine to methionine? (A) Biotin and pantothenic acid (B) Vitamin B12 and folic acid (C) Vitamin B1 and Vitamin B2 (D) Vitamin D and vitamin K

19. Which of the following matching among (a) the name of vitamin, (b) the major form of vitamer found in human serum, and (c) the biochemical function of that vitamin is correct? (A) (a) vitamin A; (b) niacin; (c) DNA synthesis (B) (a) vitamin E; (b) α-tocopherol; (c) chain-breaking antioxidant (C) (a) vitamin B1; (b) pyridoxal-5'-phosphate; (c) vision (D) (a) vitamin C; (b) retinol; (c) blood clotting

20. Which of the following statement is   not   correct about the electron carriers in the mitochondrial electron transport chain? (A) Iron-sulfur proteins transfer single electrons but not protons. (B) Cytochrome c is localized within the mitochondrial intermembrane space. (C) The oxidized form of coenzyme is also named as ubiquinol-10. (D) Cytochrome b is part of Complex III.

21. Which of following metabolites can phospholipase C convert phosphatidylinositol 4,5-bisphosphate to? (A) diacylglycerol + inositol + phosphate; (B) diacylglycerol + inositol triphosphate; (C) glycerol + inositol + phosphate; (D) phosphatidyl glycerol + inositol + phosphate 

22. What is the metabolite A in the above diagram? (A) β-ketoglutarate; (B) α-ketoglutarate; (C) α-glutarate; (D) valerate

23. What is the enzyme B catalyzing the conversion of metabolite A to succinyl-CoA? (A) valerate decarboxylase; (B) α-ketoglutarate dehydrogenase; (C) β-ketoglutarate dehydrogenase; (D) α-glutarate dehydrogenase

24. What is the enzyme C catalyzing the conversion of succinate to fumarate? (A) fumarate hydrogenase; (B) succinase; (C) succinate dehydrogenase; (D) succinate convertase

25. What is the metabolite D in the above diagram? (A) Malonate; (B) α-hydroxy-malonate; (C) n-hydroxy-succinate; (D) malate

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