17. In eukaryotic replication, the RNA primers are degraded by:
(A)the 5’ to 3’ exonuclease activity of pol ä
(B) DNA ligase
(C) Helicase
(D)FEN-1 and RNase H1

18. Which of the following correctly describes a difference between RNA & DNA polymerases? (A)RNA polymerases usually do not need a template, while DNA polymerases do. (B) DNA polymerases usually require a primer (i.e., they can only continue a strand, not start one), while most RNA polymerases do not. (C) RNA polymerases usually synthesize introns, while DNA polymerases synthesize cistrons. (D)RNA polymerases polymerize 5' → 3', while DNA polymerases polymerize 3' → 5'. (E) None of these

19. At what point does the sigma (σ) subunit of RNA polymerase released from the core enzyme? (A)Prior to the incorporation of any nucleotides into an RNA strand. (B) After transcription begins and about 10 nucleotides have been added to the RNA chain. (C) Just prior to chain termination. (D)Never； it is an intrinsic part of the core enzyme. (E) After RNA polymerase discovers an inverted repeat

複選題20. The promoter site is (A)the start site for transcription in DNA (B) the binding site for regulatory proteins that stimulate transcription (C) the general region of DNA downstream from the start site (D)the site on DNA at which RNA polymerase binds to initiate transcription (E) None of these

21. Which of the conditions would result in the greatest amount of transcription of the lac operon? (A) (B)(C) (D)

22. CREB contains the following structural motif: (A)Helix-turn-helix (B) β−barrel (C) Zinc finger (D)Leucine zipper

23. The eukaryotic TATA-binding protein (TBP) functions in a manner similar to (A)sigma (σ) factor in E. coli. (B) rho (ρ) factor in E. coli. (C) lac I in E. coli. (D)CAP in E. coli.

24. Capping of eukaryotic mRNA (A)occurs at the 5' end. (B) occurs at the 3' end. (C) occurs at both ends. (D)doesn't occur at all.

25. Polyadenylation of eukaryotic mRNA (A)occurs at the 5' end. (B) occurs at the 3' end. (C) occurs at both ends. (D)doesn't occur at all.

26. The sequences in eukaryotic DNA known as introns are (A)those included in the final sequence of messenger RNA (B) the intervening sequences not expressed in the final sequence of messenger RNA (C) the binding sites for DNA polymerase (D)the binding sites for RNA polymerase

27. Which of the following codons does not code for an amino acid? (A)AUG (B) UAA (C) CAU (D)GUU (E) All of these code for an amino acid

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