21. What enzyme breaks apart the hydrogen bonds between two strands of DNA? (A) Polymerase I. (B) Polymerase III. (C) Ligase. (D) Helicase. (E) Okazaki.

21. What enzyme breaks apart the hydrogen bonds between two strands of DNA?
(A) Polymerase I.
(B) Polymerase III.
(C) Ligase.
(D) Helicase.
(E) Okazaki.

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第26至50題每題2.5分 26. Sam is trying to separate a protein mixture containing three proteins A, B, C. Protein A: homotetramer, Mr = 120,000, pI =6； Protein B: monomer, Mr = 80,000, pI=7； Protein C: monomer, Mr = 13,000, pI=9. Please choose the correct one from the following descriptions: (A) If Sam dissolves the mixture in 0.1 M Tris-HCl (pH 7) and uses SDS-PAGE to separate the mixture, the protein bands in the gel from top to bottom should be B-A-C. (B) If Sam dissolves the protein mixture in 20 mM phosphate buffer (pH 8) and loads it to an anion exchange column equilibrated with the same buffer, only protein C can bind to the column. (C) If Sam dissolves the mixture in 0.1 M Tris-HCl (pH 7) and uses size exclusion column to separate the mixture, protein C will be first eluted. (D) If Sam dissolves the mixture in 0.1 M Tris-HCl (pH 7) and uses size exclusion column to separate the mixture, the elution sequence is B-A-C. (E) If Sam dissolves the protein mixture in 20 mM phosphate buffer (pH 7) and loads it to a cation exchange column equilibrated with the same buffer, only protein A can bind to the column.

21. What enzyme breaks apart the hydrogen bonds between two strands of DNA?
(A) Polymerase I.
(B) Polymerase III.
(C) Ligase.
(D) Helicase.
(E) Okazaki.

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相關試題

相關試卷

21. What enzyme breaks apart the hydrogen bonds between two strands of DNA?(A) Polymerase I.(B) Polymerase III.(C) Ligase.(D) Helicase.(E) Okazaki.

詳解 (共 1 筆)

相關試題

相關試卷

21. What enzyme breaks apart the hydrogen bonds between two strands of DNA?
(A) Polymerase I.
(B) Polymerase III.
(C) Ligase.
(D) Helicase.
(E) Okazaki.