31. Which of the following molecules is able to translate proteins?
(A) DNA polymerase I
(B) Proteinase K
(C) RNA polymerase II
(D) Ribosome

32. Which of the following statements is   NOT   true? (A) Histones help compact chromosomes (B) Histones help regulate gene expression in prokaryotes (C) Histones have N-terminal tails that can be covalently modified (D) Histones exist as octamers

33. Which of the following techniques   CANNOT   be used to test the loss-of-function phenotype of a gene? (A) Reporter construct (B) RNAi (C) Knock-out animal (D) Radiation induced chromosomal deletion

34. Which of the following components does   NOT   participate in protein degradation? (A) Ubiquitination (B) Proteasome (C) E3 ligase (D) Lysozyme

35. Which of the followings is   NOT   a function of carbohydrates? (A) They are the catalytic components of enzymes. (B) They play key roles in processes that take place on the surface of cells. (C) They are used in structural roles. (D) They are the major energy sources.

36. Which amino acid has the one-letter symbol E? (A) Lysine (B) Phenylalanine (C) Histidine (D) Glutamic acid

37. Which of the following amino acids contain sulfur? (A) Cysteine and lysine (B) Cysteine and methionine (C) Arginine and methionine (D) Cysteine and isoleucine

38. Which of the following strategies can be used for identifying the binding site of transcription factors within promoter region? (A) DNA microarray (B) Southern hybridization (C) DNA footprinting (D) Dot blotting

39. Which of the following statements is   CORRECT   for the purification of a biological molecule? (A) The protein G is used to purify nucleic acid and lipids. (B) Gel-filtration chromatography is a separation method for discriminating protein hydrophobicity. (C) Alteration of pH value or ion strength cannot interrupt the protein-ligand binding in the affinity purification. (D) The affinity chromatography uses the specific binding properties of a protein to purify the desired protein.

40. Which of the following strategies can be used for determining protein half-life? (A) Mass spectrometry (B) Two-dimensional electrophoresis (C) Pulse-chase labelling (D) Western blotting

41. Post-translational modification is important for protein activity. Which of the following modifications is involved in the covalent linkage between two amino acids? (A) Ubiquitination (B) Glycosylation (C) Phosphorylation (D) Acetylation

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