35. Successive base pairs in B-DNA show a rotation with respect to each other (so-called helical twist) of
(A) 34°,
(B) 36°,
(C) 38°,
(D) 32°,
(E) 40°.

36. If the rate constant for the enzyme catalyzed reaction is 1 x 105/sec and the rate constant for the uncatalyzed reaction is 2 x 10/sec, the catalytic power of the enzyme is: (A) 2, (B) (C) (D) 5 x 109, (E)

37. All of the following statements are true about the relationships between [S], and EXCEPT: (A) As [S] is increased, v approaches the limiting value, , (B) = /2, (C) The rate of the reaction, v, follows a first order rate equation v = K'[A] and K' = (D) The rate of product formed, v, is at when [S] ＞＞ , (E) assist in finding the rate of the enzyme catalyzed reaction only if the reaction is irreversible.

38. In uncompetitive inhibition: (A) I combines only with ES, (B) I combines only with E, (C) I combines with E and ES, (D) I combines with EP, (E) none of the above.

39. Formation of the ES complex results in (A) entropy gain, (B) enthalpy gain, (C) entropy loss, (D) enthalpy loss, (E) none of the above.

40. Which statement is correct about the Michaelis-Menten constant, , for the kinetic mechanism below?(A) It is numerically equal to the substrate concentration required to achieve one half the maximum velocity, (B) It's defined as ,(C) It is approximately equal to the dissociation constant for the enzyme-substrate complex to E + P, (D) The value of is constant for an enzyme regardless of the specific substrate molecule used to determine it, (E) Its numeric value has the units of . 

41. What is nitrogen fixation? (A) the reduction of , (B) the formation of from N2 gas, (C) the oxidation of to N2, (D) the oxidation of to  (E) the formation of .

42. What reaction does glutamate dehydrogenase (GDH) catalyze? (A) the reductive amination of a-ketoglutarate to yield glutamate, (B) phosphorylation of carbamate to yield carbamoyl-phosphate, (C) the amidation of the y-carboxyl group of glutamate to form glutamine, (D) the deadenylation of glutamine synthetase, (E) the adenylation of glutamine synthetase.

43. All of the following act in feedback inhibition of glutamine synthetase (GS) in prokaryotes EXCEPT? (A) AMP, (B) glucosamine-6-phosphate, (C) histidine, (D) proline, (E) CTP.

44. All of the following are characteristics of phenylketonuria EXCEPT? (A) excretion of phenylpyruvate, (B) air oxidation causes urine to turn dark on standing, (C) treated by putting patient on a diet low in phenylalanine, (D) untreated patients suffer severe mental retardation, (E) deficiency or defect in phenylalanine hydroxylase.

45. All are characteristics of xanthine oxidase EXCEPT? (A) present in large amounts in liver, intestinal mucosa and milk, (B) uses molecular oxygen and produces H2O2, (C) involved in production of urea, (D) possesses an electron-transport chain with FAD, Fe-S cluster and molybdenum cofactor (E) all are true.

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