37. Which of the following enzyme does not take part in the TCA cycle?
(A) Citrate synthase
(B) Malate dehydrogenase
(C) Aconitase
(D) Pyruvate dehydrogenase
(E) Succinate dehydrogenase

43. Which of the following enzyme does not take part in the TCA cycle? (A) Citrate synthase (B) Malate dehydrogenase (C) Aldolase (D) Aconitase (E) Fumarase

38. Which of the following statements about the reactions of glycolysis is correct? (A) glucose-6-phosphate is split into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. (B) fructose 1,6-bisphosphate is split into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. (C) fructose-6-phosphate is split into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. (D) glucose-6-phosphate is isomerized to fructose 1,6-bisphosphate. (E) glucose 2,6-bisphosphate is split into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate.

39. All are true for translation in eukaryotic cells EXCEPT: (A) transcription and translation are spatially separated. (B) mRNAs are monocistronic. (C) transcription occurs in the nucleus. (D) primary transcripts undergo processing to mature mRNAs. (E) all are true.

40. Most eukaryotic RNA consists of coding regions, called _______, and noncoding regions, called _______. (A) introns； exons (B) exons； introns (C) spliceons； codons (D) codons; spliceons (E) introns; codons

41. The adapter molecule that interacts specifically with both nucleic acids and amino acids is: (A) rRNA. (B) tRNA. (C) mRNA. (D) ssRNA. (E) all are true.

複選題42. Which of the following statements is true regarding ribosomes? (A) they are found in the cytosol and nucleus of all cells (B) prokaryotic ribosomes consist of a 30S and a 50S subunit while eukaryotic ribosomes consist of a 60S and an 80S subunit (C) ribosomes consist of both protein and ribonucleic acid with the majority of the mass from the protein component (D) since the peptidyl transferase activity is performed by RNA, it can be considered a ribozyme (E) all are true

43. Which of the following is an irreversible post-translational modification? (A) Palmitoylation (B) Proteolysis (C) Sumoylation (D) Glycolysis (E) Glycosylation

44. Which of the following compound is suitable for Nick translation to label the substrates? (A) alpha-[32P]-СТР (B) gamma-[32P]-UTP (C) S-Adenosyl-methionine (D) S-Adenosyl-L-homocysteine (E) L-[35S]-Methionine

45. To study the function of a phospho-protein, which of the amino acid substitution is commonly used in the laboratory? (A) Alanine substituted by Glycine (B) Lysine substituted by Arginine (C) Leucine substituted by Isoleucine (D) Glutamic acid substituted by Aspartic acid (E) Threonine substituted by Glutamic acid

46. Which of the following description for Polymerase Chain Reaction is not true? (A) The length of the primers controls amplification specificity. (B) The Tm of the primers controls amplification specificity. (C) High Mg2+ concentration in the reaction increases the amplification specificity. (D) The Klenow fragment of DNA polymerase I retains the 5'→3' polymerase activity and the 3'→5' exonuclease activity. (E) The Klenow fragment of DNA polymerase I loses its 5'→3' exonuclease activity.

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