4. Which of the following promotes the biosynthesis of fatty acid?
(A) Glucagon
(B) Activation of AMPK
(C) High levels of citrate
(D) Fatty acyl CoA

5. Which is the target for non-steroidal anti-inflammatory drugs (NSAID)? (A) Arachidonic acid (B) PGH synthase (C) hydrolase (D) TxA synthase

6. Which of the following is not involved in the regulation of cholesterol biosynthesis by the levels of cholesterol inside the cell? (A) The degradation of HMG CoA reductase (B) The synthesis of LDL receptor (C) The synthesis of HMG CoA reductase (D) The transport of LDL receptor to plasma membrane

7. Of the following functions, the glycoproteins and glycolipids of animal cell membranes are most important for (A) facilitated diffusion of molecules down their concentration gradients. (B) active transport of molecules against their concentration gradients. (C) maintaining the integrity of a fluid mosaic membrane. (D) the ability of cells to recognize like and different cells. 

8. In DNA sequencing by the Sanger (dideoxy) method: (A) the template DNA strand is radioactive. (B) radioactive dideoxy ATP is included in each of four reaction mixtures before enzymatic synthesis of complementary strands. (C) specific enzymes are used to cut the newly synthesized DNA into small pieces, which are then separated by electrophoresis. (D) the role of the dideoxy CTP is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.

9. When the thymine analog, 5-bromouracil, incorporates into DNA normallyoccupied by thymine, what point mutation can occur? (A) T-A to A-T (B) T-A to C-G (C) C-G to T-A (D) both （B）and（C）are correct

10. The phosphodiester bond that joins adjacent nucleotides in DNA: (A) is positively charged. (B) associates ionically with metal ions, polyamines, and proteins. (C) is susceptible to alkaline hydrolysis. (D) links C-3 of deoxyribose to N-1 of thymine or cytosine.

11. Which of the following is not true of all naturally occurring DNA? (A) Deoxyribose units are connected by 3',5'-phosphodiester bonds. (B) The amount of A always equals the amount of T. (C) The ratio A+T/G+C is constant for all natural DNAs. (D) The two complementary strands are antiparallel.

12. When double-stranded DNA is heated at neutral pH, which change does not occur? (A) The absorption of ultraviolet (260 nm) light increases. (B) The covalent N-glycosidic bond between the base and the pentose breaks. (C) The hydrogen bonds between A and T break. (D) The viscosity of the solution decreases.

13. Which of these statements about the regulation of cholesterol synthesis is not true? (A) Cholesterol acquired in the diet has essentially no effect on the synthesis of cholesterol in the liver. (B) Failure to regulate cholesterol synthesis predisposes humans to atherosclerosis. (C) High intracellular cholesterol stimulates formation of cholesterol esters. (D) Insulin stimulates HMG-CoA reductase.

14. Which of the following is not true of the reaction catalyzed by glutamate dehydrogenase? (A) It is similar to transamination in that it involves the coenzyme pyridoxal phosphate (PLP). (B) is produced. (C) The enzyme can use either as a cofactor. (D) The enzyme is glutamate-specific, but the reaction is involved in oxidizing other amino acids.

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