5. Which of the following four fatty acids has the lowest melting point?
(A) CH₃CH₂CH₂CH₂CH₂COOH
(B) CH₃CH₂CH₂CH₂CH₂CH₂CH₂COOH
(C) CH₃CH₂CH₂CH₂CH₂CH₂CH₂CH₂COOH
(D) CH₃CH=CHCH₂CH₂COOH

6. In DNA sequencing, fragments to be analyzed are produced by (A) acid hydrolysis (B) base hydrolysis (C) selective interruption of DNA synthesis (D) exposure to ³²P

7. The following enzymes of glycolysis are all involved in regulation of the pathway, except (A) Hexokinase (B) Aldolase (C) Phosphofructokinase (D) Pyruvate kinase

8. Which of the following inhibitors binds to the enzyme at a site other than the active site? (A) noncompetitive inhibitor (B) competitive inhibitor (C) irreversible inhibitor (D) all of the above

9. Which piece of DNA will have the higher Tm (if both are heated under the same experimental conditions)? (A) 30% cytosine plus guanine will have the higher Tm. (B) 50% cytosine plus guanine will have the higher Tm. (C) Their Tm's will be the same. (D) There's no way to predict for this information

10. Electron flow in the mitochondria follows this pathway: (A) Complex I → complex II → complex III → complex IV (B) Complex IV → complex III → complex II → complex I (C) Complex I → complex III → complex IV (D) Complex II → complex III → complex IV (E) Both complex I → III → IV and complex II → III → IV

11. Which of the following terms describes ATP synthesis in mitochondria? (A) substrate-level phosphorylation (B) oxidative phosphorylation (C) photophosphorylation (D) None of them

12. Which of the following modifications is likely to happen to the mRNA in a eukaryotic cell? (A) removal of intervening sequences (introns) (B) capping of the 5' end (C) addition of a poly-A tail to the 3' end (D) All of the above occur in eukaryotic cells (E) None of the above occur in eukaryotic cells

13. Which of the following is correct concerning the differences between hemoglobin and myoglobin? (A) Both hemoglobin and myoglobin are tetrameric proteins. (B) Hemoglobin exhibits cooperative binding of O₂ while myoglobin does not. (C) Hemoglobin exhibits a hyperbolic O₂ saturation curve while myoglobin exhibits a sigmoid shaped curve. (D) Hemoglobin exhibits a higher degree of O₂ saturation at all physiologically relevant partial pressures of O₂ than does myoglobin.

14. Given the rate law, rate = k[A][B], the overall reaction order is (A) zero (B) one (C) two (D) cannot be determined

15. What does amphipathic mean? (A) having both positive and negative charges (B) having both acid and base properties (C) having both hydrophilic and hydrophobic regions (D) having two stereoisomers

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