5. Which of the following methods is NOT used for gene-editing?
(A) Targeted recombination by Cre-loxP
(B) Homologous recombination
(C) CRISPR-Cas9
(D) Sequence-specific zinc-finger nucleases

6. Which of the following methods is used to quantitate newly synthesized proteins? (A) Nuclear run-on assay (B) Pulse-labeling assay (C) Polysome profiling assay (D) Western blotting assay

7. Which of the following materials can be used to cause transient knockdown of a gene? (A) Small interfering RNA (siRNA) (B) MicroRNA (miRNA) (C) Antisense oligonucleotides (ASO) (D) All of the above

8. Which of the following statements about non-coding RNA (ncRNA) is true? (A) is an RNA that is translated into a protein (B) includes transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs) (C) Many of the essential and abundant ncRNAs are involved in transcription (D) None of the above

9. Which of the following statements about epigenetics is true? (A) is the study of heritable phenotype changes that involve alterations in the DNA sequence (B) Epigenetic changes are not transmitted to the organism's offspring (C) includes DNA methylation and histone modification (D) None of the above

10. Which one of the DNA elements is not required for a chromosome to be stably maintained in cells? (A) origin of replication (B) centromere (C) primer (D) telomere

11. Chromatin consists of DNA and histone proteins. DNA and histone has strong attraction for each other because: (A) DNA is positively charged and histones are negatively charged. (B) both DNA and proteins are hydrophobic. (C) DNA is negatively charged and histones are positively charged. (D) like substances share common charges.

12. Which of the following is the least important element in plasmid DNA for making recombinant DNA: (A) a selection marker (B) DNA replication origin (C) multiple cloning sites (D) lac operon sequence for gene expression

複選題13. You would like to clone a gene by PCR. The sequence of the two ends of the coding strand for the gene is provided below. ATGAAATCTACGTTTCAC........................CCCCCAGTACCCCCCCTTA Which of the following pairs of primers would direct the amplification of this gene? All sequences are written from 5' to 3'. (A) ATGAAATCTACGTTTCAC; CCCCAGTCСССССТТА (B) ATGAAATCTACGTTTCAC; TAAGGGGGGTACTGGGGG (C) TACTTTAGATGCAAAGTG; ATTCCCCCCATGACCCCC (D) GTGAAACGTAGATTTCAT; ATTCCCCCCATGACCCCC

14. Which research topic/area was the awarded Nobel Prize in Physiology or Medicine in 2018? (A) cancer therapy by inhibition of negative immune regulation (B) molecular mechanisms controlling the circadian rhythm (C) mechanisms for autophagy (D) novel therapy against infections caused by roundworm parasites

15. The segment of the DNA molecule where messenger RNA synthesis begins is called the... (A) promoter region; (B) sigma factor; (C) transcription terminator; (D) polymerase.

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