9. Which of the followings is the most proper technique to determine the immature cell populations in patients with acute lymphocytic leukemia?
(A) Hemagglutination
(B) ELISA
(C) Flow cytometry
(D) Western blotting

10. Which of the following shoulds be free from bacteria in a healthy individual? (A) Nose (B) Skin (C) Mouth (D) Cerebrospinal fluid

12. Lysozyme and the antibiotic penicillin have similar mechanisms which causing damage to the bacterial (A) Chromosome DNA (B) Ribosome (C) Cell wall (D) Cell membrane

13. Which of the following DNA viruses undergoes genome replication via reverse transcription? (A) Adenovirus (B) Herpes simplex virus (C) Poxvirus (D) Hepatitis B virus

複選題14. Regarding bacteriophages, which of the followings is correct? (A) Lytic bacteriophages never undergo lysogenic cycle (B) Lysogenic bacteria strain can always generate bacteriophage particles upon induction (C) Bacteriophages exhibit host specificity (D) Bacteriophages bring no advantages to their hosts

15. Which of the following reactions does   NOT   require DNA polymerase? (A) PCR (B) In Vitro transcription and translation (C) DNA replication (D) DNA homologous recombination

16. Polyadenylation (polyA tail) is usually found in which type of RNA molecules? (A) rRNA (B) tRNA (C) siRNA (D) mRNA

17. Sir Frederick Sanger is a British scientist who was awarded Nobel Prize twice. Which of the following statements of him is   NOT   true? (A)He determined the structure of Insulin. (B)He developed a DNA-sequencing method. (C)He established the hybridoma technology to produce monoclonal antibodies. (D)Some of his graduate students also received the Nobel Prize

18. Please select the appropriate primers to amplify only the coding region of the following gene   EXCLUDING   the stop codon.5' GCTCGATGGATCCAAACACTGTGTCAAGCTTTCAGGACATACTGCTGAGGATGTCAAAAATGC AGTTGGAGTCCTCATCGG AGGACTTGAATGGAATGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAG AAGCAGTAATGAGAATGGGAG ACCTCCACTCACTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTCAGAAGTTTGA AGAAATAAGATGGTTGATTGAAG AAGTGAGACACAAACTGAAGATAACAGAGAATAGTTTTGAGCAAATAACATTTATGCAAGCC TTACATCTATTGCTTGAAGTGG AGCAAGAGATAAGAACTTTCTCGTTTCAGCTTATTTAG 3' (A)Primer 1: 5' GCTCGATGGATCCAAACACTGTGTC 3'; Primer 2: 5' CTTTCTCGTTTCAGCTTATTTAG 3' (B) Primer 1: 5' ATGGATCCAAACACTGTGTCAAGC 3'; Primer 2: 5' CTTTCTCGTTTCAGCTTATTTAG 3' (C) Primer 1: 5' GCTCGATGGATCCAAACACTGTGTC 3'; Primer 2: 5' CTAAATAAGCTGAAACGAGAAAGTTC 3' (D)Primer 1: 5' ATGGATCCAAACACTGTGTCAAGC 3'; Primer 2: 5' AATAAGCTGAAACGAGAAAGTTC 3'

19. The consensus sequence for poly（Ａ） addition is (A) The site of poly（Ａ） tail addition (B) AAUAAA (C) Downstream of the cleacage site (D) None of the above

20. Alternative splicing can result in which of the following gene modifications,   EXCEPT   (A) Production of different mRNA molecules from the same gene (B) Production of different proteins from the same gene (C) Production of same peptide domain (D) Production of two or more proteins with different functional domains

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