17. Which of these can be synthesized by plants but not by humans?
(A) Phosphatidylcholine.
(B)
(C) Pyruvate.
(D) Stearate (18:0).
(E) Palmitate (16:0).

18. Transport of fatty acids from the cytoplasm to the mitochondrial matrix requires: (A) ATP, coenzyme A, and hexokinase. (B) ATP, coenzyme A, and pyruvate dehydrogenase. (C) carnitine, coenzyme A, and hexokinase. (D) ATP, carnitine, and coenzyme A. (E) ATP, carnitine, and pyruvate dehydrogenase.

19. What is the correct order of function of the following enzymes for β oxidation? 1. β-Hydroxyacyl-CoA dehydrogenase; 2. Thiolase; 3. Enoyl-CoA hydratase; 4. Acyl-CoA dehydrogenase (A) 1, 2, 3, 4. (B) 4, 2, 3, 1. (C) 4, 1, 3, 2. (D) 4, 3, 1, 2. (E) 1, 4, 3, 2.

20. Which compound is an intermediate of the β oxidation of fatty acids? (A)(B) (C) CH3-CH2-CO-CH2-OH. (D) CH3-CH2-CO-CO-S-CoA. (E) CH3-CO-CH2-CO-S-CoA.

21. Posttranslational glycosylation of proteins is inhibited specifically by: (A) ampicillin. (B) cycloheximide. (C) tunicamycin. (D) puromycin. (E) streptomycin.

22. The PCR reaction mixture does not include: (A) DNA containing the sequence to be amplified. (B) heat-stable DNA polymerase. (C) DNA ligase. (D) oligonucleotide primer(s). (E) all four deoxynucleoside triphosphates.

23. In DNA sequencing by the Sanger (dideoxy) method: (A) the role of the dideoxy CTP is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands. (B) radioactive dideoxy ATP is included in each of four reaction mixtures. (C) radioactive labeling is not required. (D) restriction enzymes are used to cut the newly synthesized DNA into small pieces. (E) the template DNA strand is radioactive.

1. Calculate the pI for the peptide which has the amino acid sequence "CRYDENTH". Use the following table as reference and assume the pKa of each residue as that of the corresponding free amino acid. The pI of the peptide is: (A) 5.125. (B) 9.125. (C) 9.24. (D) 3.95. (E) 8.035.

2. Which of the following best represents the backbone arrangement of two peptide bonds? (A) Ca-N-Ca-C-Ca-N-Ca-C (B) Ca-N-C-C-N-Ca (C) C-N-Ca-Ca-C-N (D) Ca-C-N-Ca-C-N (E) Ca-Ca-C-N-Ca-Ca-C

複選題3. Emma wants to analyze the sequence of the following peptide "GHYEFLLCRGDENTTCLNKM". She uses several agents to treat the sample. Which of the following description is       correct      ? (A) If she uses phenylisothiocynate, she can get the information for the first residue only. (B) If she uses trypsin, she can get the peptide "RGDENTTCLN". (C) In order to know whether this peptide has disulfide bond, she can treat the sample with or without cyanogen bromide (CNBr) before trypsin digestion and compare the result. If cyanogen bromide makes no difference, it suggests that no disulfide bond formation in this peptide. (D) If she uses chymotrypsin, she can get the peptide "EFLLCRGDENTTCLNKM". (E) If she uses pepsin, she can get the peptide "EFLLCRGD".

4. Which one is       correct      ? (A) D-glucose and D-galactose are different on C2. (B) α-D-glucose and β-D-glucose are different on C4. (C) D-glucose and D-glucosamine are different on C2. (D) D-glucose and D-glucuronic acid are different on C1. (E) D-glucose and D-glucuronic acid are different on C2.

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