20. The following enzymes involved in the citric acid cycle are all reversible except
(A) Succinyl-CoA synthetase
(B) Succinic dehydrogenase
(C) Fumarase
(D) Citrate synthase
(E) None of the above

21. Which of the following statements about aromatic amino acids is correct? (A) All are strongly hydrophilic. (B) Histidine's ring structure results in its being categorized as aromatic or basic, depending on pH. (C) On a molar basis, tryptophan absorbs more ultraviolet light than tyrosine. (D) The major contribution to the characteristic absorption of light at 280 nm by proteins is the phenylalanine R group. (E) The presence of a ring structure in its R group determines whether or not an amino acid is aromatic.

22. Proteins may be separated according to size by (A) Isoelectric focusing (B) Affinity chromatography (C) Ion exchange chromatography (D) Molecular exclusion chromatography (E) Reverse phase HPLC

23. Hemoglobin and myoglobin both have, are, can, or do all of the following except (A) highly a helical (B) bind one molecule of heme per globin chain (C) subunits that provide hydrogen bonds to and nonpolar interaction with other subunits (D) bind heme in a hydrophobic pocket (E) bind one O2 per heme

24. A protein has one transmembrane domain composed entirely of a-helical secondary structure. Which of the following amino acids would you expect to find in the transmembrane domain ? (A) Proline (B) Glutamate (C) Lysine (D) Leucine (E) Arginine

25. How Ca++ can increase the enzymatic activity of phosphorylase for glycogen metabolism? (A) Ca++ increases the activity of calmodulin-dependent protein kinase and then activate the enzymatic activity of phosphorylase (B) Ca++ increases the activity of calmodulin-dependent phosphatase and then activate the enzymatic activity of phosphorylase (C) Ca++ increases the activity of calmodulin-dependent protein kinase and then regulates glycogen synthase kinase activity, leading to up-regulation of phosphorylase activity (D) Ca++ binds to the 8 subunit of the phosphorylase kinase and then promotes the activity of phosphorylase (E) Ca++ inhibits the protein phosphatase 1 and increases increases the activity of calmodulin-dependent phosphatase and then the enzymatic activity of phosphorylase.

26. Which following condition we cannot observe any glucosuria syndrome? (A) loss of function of pancreatic ẞ cells (B) liver damage (C) insulin increases glucose tolerance (D) inherited defects in the kidney (E) none of the above.

27. In human body what compound cannot be used for glycine biosynthesis? (A) choline (B) alanine (C) glyoxylate (D) serine (E) none of the above.

28. What following enzyme can generate ammonium (NH3)? (A) uroporphyrinogen I synthase (B) uroporphyrinogen II synthase (C) uroporphyrinogen III synthase (D) coproporphyrinogen oxidase (E) protoporphyrinogen oxidase.

29. Which one below is not the component of membrane? (A) Lipid (B) Protein (C) Cholesterol (D) Sterol (E) None of above all

30. Phosphatidylinositol (PtdIns) is a small lipid to regulate diverse biological function. The activity/function of PtdIns can be modulated by kinases and phosphatases. The kinases-mediated phosphorylation is happening on hydroxyl positions of the inositol ring. Which position below is not the site for phosphorylation? (A) 3 hydroxyl position (B) 4 hydroxyl position (C) 5 hydroxyl position (D) 3 and 4 hydroxyl positions (E) 6 hydroxyl position

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