7. Which of the following RNAs is the least abundant in a typical cell?
(A)mRNA
(B) rRNA
(C) snRNA
(D)tRNA

8. The following types of RNA are common to all organisms, except: (A)mRNA (B) rRNA (C) snRNA (D)tRNA (E) All types are found in all organisms.

9. The following enzyme is responsible for the bulk of DNA synthesis during replication. (A)DNA Polymerase I (B) DNA Polymerase II (C) DNA Polymerase III (D)DNA Polymerases IV (E) All four can make lots of DNA rapidly.

10. In E. coli, (A)the leading strand is synthesized in one piece while the lagging strand is synthesized discontinuously. (B) the leading strand is synthesized discontinuously while the lagging strand is synthesized in one piece. (C) both the leading and lagging strands are synthesized in one piece. (D)both the leading and lagging strands are synthesized discontinuously.

11. Which of the following activities does E. coli DNA polymerase III lack? (A)5' → 3' polymerase (B)5' → 3' exonuclease (C)3' → 5' exonuclease (D)E. coli DNA polymerase III has ALL of the above activities.

12. The primer for in vivo DNA replication is: (A)The 3' hydroxyl of the preceding Okazaki fragment. (B) A short piece of RNA. (C) A nick made in the DNA template. (D)A primer is not always required for DNA replication. (E) All of these are true.

13. Which of the following characteristics is not associated with E. coli primase? (A)it synthesizes the RNA primer in DNA replication (B) it synthesizes a primer with a free 3'−OH end (C) it is essential for DNA replication (D)it is essential for RNA replication

14. Which of the activities of DNA Polymerase I is most important in removing the primer? (A)Polymerase activity. (B)Ability to nick intact double stranded DNA. (C)5' → 3' exonuclease. (D)3' → 5' exonuclease. (E) None of these is used for primer removal.

15. The enzyme that attaches the Okazaki fragments together is called (A)ligase. (B) primase. (C) DNA polymerase I (D)DNA polymerase III

16. Replication of eukaryotic DNA (A)must occur faster than replication of prokaryotic DNA (B) must be controlled to coordinate with the cell cycle (C) takes place during mitosis (D)takes place twice during each cell cycle

17. In eukaryotic replication, the RNA primers are degraded by: (A)the 5’ to 3’ exonuclease activity of pol ä (B) DNA ligase (C) Helicase (D)FEN-1 and RNase H1

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